利用药用野生稻C基因组C0t-1DNA比较分析宽叶野生稻CCDD基因组.pdfVIP

2010, 11( 5): 589592, 599 JournalofP lantGenetic Resources 利用药用野生稻 C基因组C0 t 1 DNA 比较分析 宽叶野生稻 CCDD基因组 吴 绮, 覃 瑞,李 刚, 刘 虹 ( /, 430074) : 以来源于 C基因组的药用野生稻的中高度重复序列 C t1 NA 为探针, 不同的洗脱严谨度下, 通过荧光原位杂交对宽叶 0 野生稻( CC )基因组进行了分析结果发现, 随着洗脱严谨度的调整, 杂交信 号呈现出较高的特异性, 主要分布 着丝粒近着丝粒 及端粒区域本文以宽叶野生稻的核型分析为基础, 比较其与二倍体药用野生稻基因组的异同, 从而进 一步探讨野生稻的进化起源 机制 : C t1 NA; ; ; 0 ComparativeAnalysis on CCDD Genome ofO . latif olia with CC C0 t 1 DNA from O . off icina lis WU Q,i QIN Rui, LIGang, LiU Hong (Eng ineering Research Centref or the Protection and Utilization of B ioresource in EthnicA rea of Sou thern China/College of Lfi e Sciences, SouthCentral Un ivers it f or N ationa lities, Wuhan 430074 ) Abstract:The highly andmoderately repetitive NA sequence CCC t1 NA ofO. off icinalis was used as probe 0 to analyseO. latfi olia genome comparatively by fluorescence in situ hybridization (FISH) in different stringency. Re sult shows that the hybridizational signals of chromosomesmainly distributed on centromere, acrocentric and telomere, showing higher specificity w ith the change of stringency relatively. In order to explore the evolutional and genetic mechanism and find out the genom ic differences between diploidO. off icinalis and polyploid O. latfi olia, comparative analysis of genomes betweenO. off icinalis andO. latfi olia was carried outbased on karyotype analysis. Key words: C t1 NA; FISH; Stringency; O. latfi olia 0 (Or za L. )(Gram ineae) (Oryzeae umortier), ,