蛋白质胶内酶切技术的研究进展.pdfVIP

生物技术通报 ·技术与方法· BIOTECHNOLOGY BULLETIN ( ) 2015, 31 1 :61-66 蛋白质胶内酶切技术研究进展 1，2 1 2 姜超   张学文  潘映红 （1. 湖南农业大学生物科学技术学院，长沙 410128 ；2. 中国农业科学院作物科学研究所 国家农作物基因资源与基因改良重大科学工程， 北京，100081） 摘 要 ： 胶内酶切是蛋白质组研究中衔接电泳分离和质谱鉴定的重要环节，对最终的蛋白质定性和定量分析结果有显著的 影响。该技术自1992 年初步建立以来，一直处于不断完善中，出现了种类繁多的改进方案。为了更有效地利用胶内酶切技术，从 凝胶脱色、杂质去除、蛋白酶切、肽段提取4 个方面归纳整理了近年来蛋白质胶内酶切技术的主要研究进展。 关键词 ： 蛋白质胶内酶切 ；样品制备 ；质谱分析 ；电泳分离；技术进展 DIO ： 10.13560/ki.biotech.bull.1985.2015.01.009 - Research Progress on Technology of In gel Protein Digestion 1，2 1 2 Jiang Chao Zhang Xuewen Pan Yinghong （1. College of Bbioscience and Biotechnology ，Hunan Agriculture University ，Changsha 410128 ；2. Institute of Crop Science ，Chinese Academy of Agricultural Sciences ，The National Key Facility for Crop Gene Resources and Genetic Improvement ，Beijing 100081 ） Abstract: In-gel digestion is the important step that connects electrophoresis separation and mass spectrometry identification in proteome research, and can significantly affect the results of qualitative and quantitative analysis of proteins. The technology was firstly proposed in 1992, and from then on, many progresses have been made and various improvement plans have been developed. In order to more effectively use the technology of in-gel digestion, the progress on the aspects of gel decoloration, impurity removal, protein digestion and peptides extraction were reviewed from the literature. Key words: in-gel