大肠杆菌K235能量代谢扰动影响聚唾液酸合成研析.pdf

生物技术通报 ·研究报告· BIOTECHNOLOGY BULLETIN ( ) 2015, 31 9 :209-217 大肠杆菌 K235 能量代谢扰动影响聚唾液酸合成的研究 闫霞  吴剑荣  郑志永  詹晓北 （江南大学生物工程学院 糖化学与生物技术教育部重点实验室，无锡 214122） 摘 要 ： 通过不同装液量、不同氧化还原状态的碳源和添加NAD+ 的前体烟酸来调控大肠杆菌胞内的能荷、还原力水平，研 究胞内核苷酸和聚唾液酸合成之间的关系。结果表明，聚唾液酸合成量越高，胞内UTP 含量越低、UMP 含量越高 ；同时，合成 UTP、CTP 消耗大量ATP 使胞内能荷水平较低。与其他碳源的发酵相比，以山梨醇为碳源时，由于胞内NADH 水平的提高使菌体 合成的聚唾液酸量最高。添加烟酸可以提高胞内的NAD+ 水平，导致胞内的氧化水平提高，从而使胞内还原产物如聚唾液酸、琥珀酸、 乳酸的产量降低甚至消失。 关键词 ： 大肠杆菌；聚唾液酸 ；能荷；核苷酸 ；氧化还原势 DOI ：10.13560/ki.biotech.bull.1985.2015.09.030 Influence of Perturbation of Energy Metabolism in Escherichia coli K235 on Biosynthesis of Polysialic Acid Yan Xia Wu Jianrong Zheng Zhiyong Zhan Xiaobei （Key Laboratory of Carbohydrate Chemistry Biotechnology of Ministry of Education ，School of Biotechnology ，Jiangnan University ， Wuxi 214122 ） + Abstract: Different medium volumes, carbon sources with varied redox states and adding nicotinic acid, and the precursor of NAD were used to adjust the intracellular energy charge and reduction of Escherichia coli in order to investigate the relationships between biosynthesis of polysialic acid and intracellular nucleotides. The results showed that the higher the production of polysialic acid was, the lower UTP was, but higher UMP was. Meanwhile, energy charge level was lower because the synthesis of UTP and CTP consumed a lot of ATP. Using sorbitol as carbon source, more polysialic acid was produced than those of fermentation with glucose, xylose and sodium gluconate due to the increase of