枯草芽孢杆菌表达系统及启动子的研究进展.pdf

生物技术通报 ·综述与专论· BIOTECHNOLOGY BULLETIN ( ) 2015, 31 2 :35-44 枯草芽孢杆菌表达系统及其启动子研究进展 余小霞  田健  刘晓青  伍宁丰 （中国农业科学院生物技术研究所，北京 100081） 摘 要 ： 枯草芽孢杆菌作为一种革兰氏阳性细菌，由于其具有非致病性、分泌蛋白能力强的特性和良好的发酵基础及生产 技术，是目前原核表达系统中表达和分泌外源蛋白的理想宿主，成为原核表达系统中的一种重要的模式菌株。而实现外源蛋白的 高效表达的关键因素之一是使用强并可控制的启动子。 目前，枯草芽孢杆菌中常用的启动子为组成型、诱导物诱导型、时期特异 性及自诱导型。详细介绍枯草芽孢杆菌表达系统以及其常用启动子的优缺点，并对克隆新的启动子的方法做了总结，旨为完善枯 草表达系统和工业生产外源蛋白奠定基础。 关键词 ： 枯草芽孢杆菌 ；表达系统 ；组成型启动子 ；诱导型启动子 DOI ：10.13560/ki.biotech.bull.1985.2015.02.005 Research Progress of Bacillus subtilis Expression System and Its Promoter Regulatory Elements Yu Xiaoxia Tian Jian Liu Xiaoqing Wu Ningfeng （Institute of Biotechnology, Chinese Academy of Agricultural Sciences, Beijing 100081 ） Abstract ： As a Gram-positive bacteria, Bacillus subtilis is an attractive host for the production of heterologous secretory proteins for several reasons ：it is non-pathogenic and the capable of secreting functional extracellular proteins directly to the culture medium, a great deal of vital information concerning large scale fermentation and production technology. One of the key factors for achieving high-level expression of heterologous proteins is the use of a strong and control promoter. In present, the promoters of Bacillus subtilis can be classified into three categories ：constitutive promoters, inducer-specific promoters and autoinducible promoters. This paper described the advantages and disadvantages of Bacillus subtilis expression system and the classification of promoters. At the same time, we summarized the methods of the amplification of new promoters, which provided a foundatio