纳米材料固定化酶研析进展.pdfVIP

生物技术通报 ·综述与专论· BIOTECHNOLOGY BULLETIN 年第 期 2013 6 纳米材料固定化酶的研究进展 1 2 1 1 高启禹  徐光翠  陈红丽  周晨妍 （1. 新乡医学院生命科学技术学院 河南省遗传性疾病与分子靶向药物重点实验室培育基地，新乡 453003 ； 2. 新乡医学院公共卫生学院，新乡 453003） 摘 要 ： 纳米材料在蛋白酶及核酶的固定化研究领域进展迅速，主要包括各种磁性纳米载体及非磁性纳米载体。 目前在固 定化纳米载体的特性、固定化方法及固定化效果上已进行了广泛探讨。综述以纳米载体的研究现状为基础，分析纳米载体固定化 酶的应用前景及纳米载体固定对酶学性质的影响，并对该技术的研究进行介绍和展望。 关键词 ： 纳米材料 固定化酶 磁性载体 非磁性载体 核酶 Research Progress of Nanoparticles for Immobilized Enzymes 1 2 1 1 Gao Qiyu Xu Guangcui Chen Hongli Zhou Chenyan （1. College of Lif e Science and Technology ，Xinxiang Medical University ，Henan Key Laboratory of Hereditary Disease and Molecular Target Drug Therapy （Cultivating Base ），Xinxiang 453003 ；2. College of Public Health ，Xinxiang Medical University ，Xinxiang 453003 ） Abstract:  Immobilization of protease and ribozyme by nanometer carrier are researched as a more useful means, including of the magnetic nanoparticle and nonmagnetic nanoparticles. Currently, the types of immobilized carrier and methods and results of nanoparticles are discussed. In this paper, we describe the current application of immobilized enzyme by nanocarrier, the effect of nanoparticles matrix to enzymatic properties and the prospect of application for the above mentioned technology were introduced, and the direction of the development of nanoparticles immobilization of enzyme was analyzed. Key words:  Nanoparticle cartie Immobilized enzymes Magnetic nanoparticles Non magnetic nanoparticles Ribozyme