二氢黄酮醇4-还原酶的生物信息学分析.pdfVIP

生物技术通报 BIO TE CHN OLOG Y B ULLETIN 2010 12 4 陈大志 周嘉裕 李萍 (, 610031) : 花色素苷是影响花色的主要色素, DFR 是花色素苷合成的关键酶采用生物信息学的方 分析已经在 Gen Bank上注册的拟南芥金鱼草兰花山茶番茄水稻矮牵牛和玉米等植物的 DFR 核酸及相应氨基酸序列, 并对其理化性 质生化功能系统发育关系和结构特征等进行预测结果表明, 金鱼草和山茶DFR 定位于叶绿体, 矮牵牛 DFR 定位于线粒 体, 山茶和矮牵牛DFR 都是跨膜蛋白DFR 拥有 一个NADB_Rossm ann superfam ily保守结构域和 oiled oil结构; 所构建的 DFR基因系统发育关系与形态学上的物种发育关系基本吻合; 螺旋和无规则卷曲是DFR 的主要二级结构元件; DFR 的空间 结构分为两 个部分, 松散 C末端和致密球状结构 : 花色素苷合成 DFR 生物信息学 B ioinform atics Analysis of D ihydroflavonol 4R eductase Chen Dazh i Zhou Jiayu Li P ing ( S outhw est J ia otong Un iv ersity, S ch oo l of L fi e S c ien ce and E ng in eering, Chengdu 610031) Abstrac:t Antho yanin is the m ain p igment to affe t flow er olor, and DFR is a key enzym e in antho yanin synthes is. In this study, DFR from A rab idop sis th a liana, A ntirrh in um m a us, B romh ead ia f inlayson ia na, C am ellia sinens is, Lyc op ersicon esculentum, O ryza sativa, P e tun ia hy brid and Z ea m ays, wh i h w ere registered in GenBank, w ere analyzed and predi ted by tools of b ioinformati s in the follow ing aspe ts: physi al and hem i al properties, b io hem i al fun tion, phylogeny, stru ture of protein. The results as follow ing: DFR from A nt irrh inum m a us and Cam e ll ia sinensis lie in hlorop last, P e tun ia hybr id DFR lo ates in m ito hondria, both ofCam el l ia s inensis and P e tun ia hy brid DFR are transm em brane proteins. DFR ontains onserved NADB_Rossm ann superfam ily dom ain and oiled oil stru tures; DFR gene phylogeny is in agreem ent w ith m orphologi al spe ies phylogeny in general; helix and random oil are prmi ary se ondary stru tural om ponen ts of DFR; the spatial st