木薯MeASR基因克隆及其表达分析.pdfVIP

生物技术通报 ·研究报告· BIOTECHNOLOGY BULLETIN ( ) 2015, 31 10 :125-130 木薯 MeASR 基因克隆及表达分析 胡伟  颜彦  韦运谢  王文泉  夏志强  卢诚  侯晓婉  彭明 （中国热带农业科学院热带生物技术研究所，海口 571101） 摘 要 ： 脱落酸- 胁迫- 成熟诱导蛋白 （Abscisic acid-stress-ripening，ASR）在植物对非生物逆境胁迫的应答过程中发挥着 重要作用。利用PCR 技术从木薯中克隆了第一个ASR 基因MeASR ，序列分析表明该基因开放阅读框 （ORF）330 bp，编码109 个 氨基酸。多序列比对和进化树分析表明该基因所编码的蛋白具有ASR 家族蛋白的保守结构域，与番茄ASR 家族蛋白SlASR4 具有 较近的亲缘关系。亚细胞定位分析表明MeASR 定位在细胞核，实时荧光定量PCR 分析表明该基因的表达显著受渗透胁迫和ABA 诱导。结果表明，MeASR 可能作为转录因子参与木薯对干旱逆境胁迫应答及ABA 信号调节。 关键词 ： 木薯 ； 基因 ；基因克隆 ；表达分析 ；亚细胞定位 MeASR DOI ：10.13560/ki.biotech.bull.1985.2015.10.021 Clone and Expression of MeASR Gene in Cassava Hu Wei Yan Yan Wei Yunxie Wang Wenquan Xia Zhiqiang Lu Cheng Hou Xiaowan Peng Ming （Institute of Tropical Bioscience and Biotechnology ，Chinese Academy of Tropical Agricultural Science ，Haikou 571101 ） Abstract:  Abscisic acid-stress-ripening induced protein（ASR）plays an important role in response to abiotic stresses. In the present study we isolated a first ASR gene designated MeASR from cassava. Sequence analysis showed that the open reading frame of MeASR gene contained 330 bp, encoding 109 amino acids. Multiple sequence alignment and phylogenetic analysis indicated that MeASR protein contained the conserved domains as ASR family had, and had a close genetic relationship with SlASR4 of tomato. Subcellular location assay showed that MeASR protein was localized in nucleus. Real-time quantitative PCR assay revealed that expression of MeASR was induced by osmotic stress and ABA treatments. These results suggested that MeASR might function as a transcription factor to involve in response to drought stress and ABA- signal