产脂肪酶嗜酸性真菌的筛选、鉴定及产酶条件优化.pdfVIP

生物技术通报 ·研究报告· BIOTECHNOLOGY BULLETIN ( ) 2015, 31 10 :171-176 产脂肪酶嗜酸性真菌的筛选、鉴定与产酶条件优化 赵军  赵淑琴  杨孝朴  刘晓丽 （甘肃农业大学动物医学院，兰州 730070） 摘 要 ： 旨在筛选获得高产脂肪酶菌株，并鉴定其种属及确定最佳产酶条件。从兰州城区附近采集富含油脂的土壤，通过 维多利亚蓝与罗丹明B 培养基进行菌株分离初筛。用改进铜皂- 分光光度法测定酶活力值进行复筛，获得一株产酶能力较强的菌 株LZ-6。经形态学观察和分子生物学鉴定，该菌株为雅致放射毛霉 （ ）。经单因素实验与正交实验确定该菌株的 Actinomucor elegans 最佳产酶条件为：酵母粉1%、橄榄油2%、MgSO ·7HO 0.05%、（NH ）SO 0.1%、K HPO 0.2%、温度30℃、pH5.0、接种量为2%、 4 2 4 2 4 2 4 发酵5 d，在此条件下，产酶活力达13.35 U/mL，比优化前提高了1.78 倍。 关键词 ： 真菌 ；脂肪酶 ；筛选；鉴定 ；优化 DOI ：10.13560/ki.biotech.bull.1985.2015.10.027 Screening and Identification of Eosinophilic Fungal Strain Producing - Lipase and Optimization of Its Lipase producing Condition Zhao Jun Zhao Shuqin Yang Xiaopu Liu Xiaoli （College of Veterinary Medicine ，Gansu Agricultural University ，Lanzhou 730070 ） Abstract:  This experiment was devoted to screen the strains of high-yield lipase, identify the genera, and define the optimal fermentation conditions. The strains were preliminary screened from greasy soil nearby Lanzhou urban area through Victoria blue and rhodamine B plates. The strains were re-screened through a copper soap spectrophotography measuring lipase activity while using emulsified olive oil as the substrate, finally a fungal strain LZ-6 with high-yield lipase was obtained. It was identified as Actinomucor elegans through morphological observation and mo