甲异靛通过tlr4-tak-nf-κb途径治疗银屑病的机制的研究.pdfVIP

药学学报 Acta Pharmaceutica Sinica 20 13, 48 (4): 503 507 ·503 · 异靛通过 TLR4-TAK-NF- B 途径治疗银屑病的机制研究 张海婧, 翼, 金 晶, 周琬琪, 陈晓光* ( 中国医学科学院、北京协和医学院 物研究所天然 物活性物质与功能国家重点实验室, 北京 100050) 摘要: 在近年来的临床实践中发现, 甲异靛对银屑病有很好的疗效, 但作用机制不明。利用脂多糖刺激 RAW264.7 细胞后, 发现甲异靛对其产生一氧化氮有明显的抑制作用。同时检测了甲异靛作用 HaCaT 细胞后对 免疫相关细胞因子及上游通路细胞蛋白的影响。结果发现, 甲异靛抑制了众多炎症相关因子的表达, 如一氧化氮 合成酶、环氧合酶2 、肿瘤坏死因子等。对上游通路 白表达进行检测后发现, 甲异靛可以相继抑制NF- B 、TAK 和 TLR4 等蛋白的表达。本研究结果显示, 甲异靛可以通过作用于 TLR4-TAK-NF- B 通路, 从而治疗或缓解银 屑病。 关键词: 甲异靛; 银屑病; 细胞因子 中图分类号: R963 文献标识码: A 文章编号: 0513-4870 (20 13) 04-0503-05 Mechanism about therapeutic effect of meisoindigo on psoriasis via down-regulation of the TLR4-TAK-NF- B pathways * ZHANG Hai-j ing, ZHANG Yi, JIN Jing, ZHOU Wan-qi, CHEN Xiao-guang (State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Ac ademy of Medical Sciences Peking Union Medical College, Beij ing 100050, China ) Abstract : Meisoindigo is an indigo natural derivative commonly used in anti-cancer therapy. In the clinical application, it was also found to have good therapeutic effect on psoriasis. In order to further understand its mechanism of action, human normal keratinocyte cell line HaCaT and RAW 264.7 were used to identify if meisoindigo could affect the inflammatory factors such as NO and other important cytokines which were highly involved in psoriasis. Our