rna-seq及道地药材的研究.pdfVIP

1650 药学学报 Acta Pharmaceutica Sinica 20 14, 49 ( 12): 1650 1657 RNA-Seq 与道地药材研究 1 1 1 1* 2* 王晓玥 , 宋经元 , 谢彩香 , 韩建萍 , 陈士林 (1. 中国医学科学院、北京协和医学院药用植物研究所, 北京 100193; 2. 中国中医科学院中药研究所, 北京 100700) 摘要: RNA-Seq 是一种新崛起的转录组学研究方法, 利用新一代测序技术能够更为准确、快速地为人们提供 更多的生物体转录信息, 已广泛应用于多种生物领域。道地药材以质优、疗效好的优势历来被各医家所推崇, 其 道地性形成的根本是其基因型在特定时空表达的 物。因此通过转录组测序来解析其生物合成机制, 已成为当 前药用植物次生代谢研究的热点。本文主要介绍了RNA-Seq 技术及其优势, 并就其在道地药材形成机制研究方 面的应用进行了讨论, 为今后该技术的研究与应用提供参考。 关键词: RNA-Seq; 道地药材; 次生代谢 中图分类号: R93 1 文献标识码: A 文章编号: 0513-4870 (20 14) 12- 1650-08 RNA-Seq and genuine traditional Chinese medicine 1 1 1 1* 2* WANG Xiao-yue , SONG Jing-yuan , XIE Cai-xiang , HAN Jian-ping , CHEN Shi-lin (1. Institute of Me dicinal Plant Development, Chinese Ac ademy of Medical Sciences and Peking Union Medical College, Beijing 100193, China; 2. Institute of Chinese Materia Medica, China Ac ademy of Chinese Medical Sciences, Beijing 100700, China ) Abstract : RNA-Sequencing (RNA-Seq) is a newly-developed method in transcriptome research, it can afford more accurate transcription information and be more quickly by using Next-generation Sequencing (NGS) technology. RNA-Seq has been widely used in various biological fields. Genuine traditional Chinese medicines (TCM), with good quality and therapeutic effect, were always praised highly and used by famous physicians. The geo-herbalism formation of TCM is based on the product of the gene expression at s