参芪抑瘤方药物血清对胃癌MKN―45细胞周期阻滞及抗侵袭转移的影响.docVIP

参芪抑瘤方药物血清对胃癌MKN―45细胞周期阻滞及抗侵袭转移的影响 　　摘要：目的 探讨不同浓度参芪抑瘤方药物血清对胃癌MKN-45细胞周期阻滞及抗侵袭转移作用的相关机制。方法 60只SPF级Wistar大鼠，随机分为对照组和参芪抑瘤方低、中、高剂量组，每组15只。参芪抑瘤方低、中、高剂量组分别给予0.25、0.50、1.00 g原药材/mL药液灌胃，空白组给予等量饮用水灌胃，每日2次，连续7 d，末次灌胃2 h后取血，分离血清。MKN-45细胞经不同浓度药物血清处理后，流式细胞术检测细胞周期，免疫组化检测COX-2、PTEN蛋白表达。结果 药物血清干预后细胞G0～G1期比例增加，S期比例减少；药物血清可降低MKN-45细胞COX-2蛋白阳性表达率，升高PTEN蛋白阳性表达率（P中国论文网 /6/view-7254757.htm 　　关键词：参芪抑瘤方；胃癌；MKN-45细胞；细胞周期；COX-2蛋白；PTEN蛋白；大鼠 　　DOI：10.3969/j.issn.1005-5304.2016.04.017 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2016）04-0064-04 　　Effects of Shenqi Yiliu Decoction Medicated Serum on Blocking Cell Cycle and Inhibiting Invasion and Metastasis of Gastric Cancer Cell Lines MKN-45 MA Chun-lin1， WU Hong-yan1，2，3， LI Hai-long1，2， CHEN Jie1， ZHANG Xuan1 （1. Gansu University of Traditional Chinese Medicine， Lanzhou 730000， China； 2. Key Laboratory of Traditional Chinese Medicine Excavation Innovation and Transformation of Gansu Province， Lanzhou 730000， China； 3. Laboratory for TCM New Products Development Engineering of Gansu Province， Lanzhou 730000， China） 　　Abstract： Objective To investigate the relevant mechanism of different concentrations of Shenqi Yiliu Decoction medicated serum on blocking cell cycle and inhibiting invasion and metastasis of gastric cancer cell lines MKN-45. Methods Sixty SPF Wistar rats were randomized into Shenqi Yiliu Decoction low-， medium-， and high-dose groups and control group， with 15 rats in each group. Low-， medium- and high-dose groups were fed with different concentrations of TCM liquid from which containing crude medicine （0.25， 0.50， 1.00 g/mL） separately for gavage. Rats in the blank group were given the same amount of drinking water for gavage， twice a day， for 7 days. 2 h after the last gavage， rat blood was taken and serum was separated. After MKN-45 cells were dealt with different concentrations of medicinal serum， FCM was used to detect cell cycle and immunohistochemistry technology was used to detect the expressions of COX-2 and PTEM proteins. R