文献阅读-2012年9月-虞艳云课件.doc

1. Baker MG, Lalonde SV, Konhauser KO, Foght JM. Role of Extracellular Polymeric Substances in the Surface Chemical Reactivity of Hymenobacter aerophilus, a Psychrotolerant Bacterium. Applied and Environmental Microbiology, Jan. 2010, p. 102–109 Abstract: Bacterial surface layers, such as extracellular polymeric substances (EPS), are known to play an important role in metal sorption and biomineralization; however, there have been very few studies investigating how environmentally induced changes in EPS production affect the cell’s surface chemistry and reactivity. Acid base titrations, cadmium adsorption assays, and Fourier transform infrared spectroscopy (FT-IR) were used to characterize the surface reactivities of Hymenobacter aerophilus cells with intact EPS (WC) or stripped of EPS (SC) and purified EPS alone. Linear programming modeling of titration data showed SC to possess functional groups corresponding to phosphoryl (pKa~6.5), phosphoryl/amine (pKa ~7.9), and amine/hydroxyl (pKa~9.9). EPS and WC both possess carboxyl groups (pKa~5.1 to 5.8) in addition to phosphoryl and amine groups. FT-IR confirmed the presence of polysaccharides and protein in purified EPS that can account for the additional carboxyl groups. An increased ligand density was observed for WC relative to that for SC, leading to an increase in the amount of Cd adsorbed (0.53 to 1.73 mmol/liter per g [dry weight] and 0.53 to 0.59 mmol/liter per g [dry weight], respectively). Overall, the presence of EPS corresponds to an increase in thenumber and type of functional groups on the surface of H. aerophilus that is reflected by increased metal adsorption relative to that for EPS-free cells. 题目：一种耐寒细菌 Hymenobacter aerophilus 的EPS在表面化学活性中的作用 细菌的表面层，如胞外聚合物（EPS），在金属吸附和生物矿化上起着重要的作用，然而很少有研究调查环境引起的EPS的变化如何影响细胞的表面化学和反应变化。酸碱滴定法，Cd 吸附曲线，傅里叶变换红外光谱（FT-IR），被用于表征具有完整 EPS 层（WC）和及剥离EPS层（SC）的 Hymenobacter aerophilus 以及纯 EPS 的细胞表面的化学反应。滴定的线性规划模型数据表明 SC 具备相应功能团体磷（PK~6.5），磷/胺（PK~7.9），胺/羟基（PK~9.9）。EPS 和 WC 都具有羧基不具备磷和胺基（