荧光法测定水中氨氮浓度的研究精要.docVIP

CHANGZHOU INSTITUTE OF TECHNOLOGY 毕 业 论 文 题目： 荧光法测定水中氨氮浓度的研究 二级学院： 理学院 专 业： 化学工程与工艺 班级： 10化学Y 学生姓名： 肖春芬 学号： 指导教师： 曹桂萍 职称： 副教授 评阅教师： 职称： 2014 年 6 月摘要 水中的氨氮可以在一定条件下转化成亚硝酸盐，如果长期饮用，水中的亚硝酸盐将和蛋白质结合形成亚硝胺，这是一种强致癌物质，对人体健康极为不利。258nm，发射波长为284nm，盐酸浓度为0.8×10-3mol/L，反应时间为4小时，氯化苄的浓度为1.8×10-4mol/L，碳酸氢钠浓度为0.03mol/L，加入β-环糊精浓度为0.012g/L，在此条件下本方法的线性范围为0.01-0.6mg/L，最低检出限为0.004mg/L。在实际水样和模拟水样中加入5mg/L氨氮标准使用溶液测其加标回收率在93%-103%之间，其精密度在0.3-6%之间。从而确定本实验方法具有检测快速、准确、灵敏度高和样品预处理简单的优势，适用于各种水样的测定。 关键词：氨氮 含量测定 荧光法 氯化苄 Abstract Ammonia water can under certain conditions into nitrite, if long-term drinking water and protein binding of nitrite to form nitrosamines, which is a potent carcinogen, is extremely detrimental to human health. Therefore, ammonia plays an important role in water and wastewater monitoring, are stations at all levels must be measured. The experimental method to select chlorinated benzene and ammonia under alkaline conditions the reaction of benzyl amine to produce a fluorescent signal, to determine the optimal experimental conditions, a sensitive and accurate fluorescence analysis. The results show that the excitation wavelength for this experiment 258nm, emission wavelength of 284nm, the hydrochloric acid concentration 0.8×10-3mol/L, the reaction time was 4 hours , the concentration of benzyl chloride was 1.8×10-4mol/L, the concentration of sodium bicarbonate 0.03mol/L, adding β-cyclodextrin concentration of 0.012g/L, under these conditions the linear range of the method is 0.01-0.6mg/L, the lowest detection limit of 0.004mg/L. Join 5mg/L of ammonia in water samples and standard analog water samples was measured using their recoveries between 93%-103% of its precision between 0.3-6%. The experimental method to determine the detection of fast, accurate, high sensitivity and simple sam