6种培养基配方要点分析.docxVIP

1、Acetobacter Medium (醋酸菌培养基)?( w. X4 x# m) q5 D% F5 t0 {Glucose (葡萄糖) 100g Yeasst extract (酵母膏) 10g?CaCO3 20g Agar (琼脂) 15g?Distilled water (蒸馏水) 1000ml Adjust (调) pH to 6.8? D??T! [+ O* [0 {适用范围：恶臭醋酸杆菌混浊变种?% Z0 Z4 u+ @9 D2 u1 w5 |2 Z, z q2、 Nutrient Agar (营养肉汁琼脂)?Pepton (蛋白胨) 5g Beef extract (牛肉膏) 30g?NaCl 5g Agar (琼脂) 15g?; j; {/ c2 y G: GDistilled water (蒸馏水) Adjust (调) pH to 7.0-7.2?1 e5 r% I) L( W E: u[Note]：When cultivation of Bacillus，5mg of to MnSO4.H2O may be added .?It is favorable to promote spore formation .?适用范围：产气气杆菌、粪产碱杆菌、蜡状芽孢杆菌、蜡状芽孢杆菌蕈状变种、地衣形芽孢杆菌、巨大芽孢杆菌、多粘芽孢杆菌、尘埃芽孢杆菌、短小芽孢杆菌、嗜热脂肪芽孢杆菌、枯草芽孢杆菌、枯草芽孢杆菌深黑变种、苏云金芽孢杆菌、苏云金芽孢杆菌蜡螟亚种（青虫菌）、苏云金芽孢杆菌戈尔斯德变种、苏云金芽孢杆菌猝倒亚种、产氨短杆菌、黄色短杆菌、谷氨酸棒状杆菌、北京棒杆菌、大肠埃希氏菌（大肠杆菌）、铜绿假单胞菌（绿脓杆菌）、凸形假单胞杆菌、荧光假单胞菌、弯曲假单胞菌、恶臭假单胞菌、假单胞杆菌、藤黄八叠球菌、亚黄八叠球菌、尿素八叠球菌、金黄色葡萄球菌、运动发酵单孢菌?0 {$ y* W. e( K7 Z K??X: A# |7 Z% `$ C6 t3、Azotobacter Medium (固氮菌培养基)?1 P% j. F, ? ^6 d aKH2PO4 0.2g K2HPO4 0.8g?MgSO4.7H2O 0.2g CaSO4.2H2O　　 0.1ｇ?Na2MoO4.2H2O Trace(微量) Yeast axtract(酵母膏) 0.5g?; `0 M% y( p5 Y+ ]) bMannitol(甘露醇) 20g FeCl3 Tract(微量)?! i: W??Y/ C! j1 u I/ SDistilled water (蒸馏水) 1000ml Agar (琼脂) 15g?Adjust (调) pH to 7.2?) F1 q8 Y6 R5 f( v适用范围：固氮菌、胶质芽孢杆菌?) `: h) N p6 Y: j5 F4、Corn Meal Medium (玉米粉培养基)?: Q a( m* T% G2 e??JMaize flour (玉米粉) 5g Peptone (蛋白胨) 0.1g?; Q m1 `# A2 s Q- {: F: IGlucose (葡萄糖) 1g Tap water (自来水) 1000ml?1 y: s3 R6 j% n7 g5 n+ {3 H; l$ V2 o[Note]:Boil the mixture in autoclave at 121℃ for 1 hr. distribute the medium into 18ⅹ18 mm tubes , each contains 10 ml of the liquid , then autoclave at 121℃ for 1 hr . again (15磅蒸煮1小时,分装入18ⅹ18毫米试管,每管深度达6厘米。15磅再次灭菌15小时。)?8 W+ A ]8 Q h0 m7 O g4 m Q f7 r??Z Z; W, @ v5、Lactic-bacteria Medium I (乳酸菌培养基 I )?* \6 y5 I* O+ X7 \0 v6 MYeast extract (酵母膏) 7.5g Peptone (蛋白胨) 7.5g?Glucose (葡萄糖) 10g KH2PO4 2g???M: y( Q- `( _7 e+ k; gTomato juice (西红柿汁) 100ml Tween (吐温) 80 0.5ml?Distilled water (蒸馏水) 900ml pH 7.0?3 _$ ?$ x6 `2 H适用范围：植物乳杆菌（胚芽乳杆菌）、嗜热乳酸链球菌?6、Lactic-bacteria Midium Ⅱ (乳酸菌培养基 Ⅱ)?