应用环磷酰胺对免疫抑制模型的建立及免疫抑制模型的检测重点解读.docVIP

应用环磷酰胺对免疫抑制模型的建立及免疫抑制模型的检测 摘要 目的：应用环磷酰胺建立小鼠免疫抑制模型并检测免疫抑制模型建立是否成功 方法：十六只小鼠分为四大组，每组四只。将四只小鼠平均分为两组，分别为对照组和免疫抑制组，第一天对两组的四只小鼠进行初次免疫，对照组小鼠分别注射0.4mlNS和0.4mlOVA-AL(OH)3，免疫抑制组小鼠分别注射0.4mlNS 和0.4mlOVA-AL(OH)3。此后分别于第8、10、12、14天给对照组小鼠注射0.4mlNS，给免疫抑制组小鼠注射100mg/kg的CTX。于第十五天对两组小鼠加强免疫，对照组小鼠分别注射0.4mlNS和0.4mlOVA-AL(OH)3，免疫抑制组小鼠分别注射0.4mlNS和0.4mlOVA-AL(OH)3，即四组分别为NS-NS组、OVA-NS组、NS-CTX组、OVA-CTX组，免疫抑制模型建立。用小鼠腹腔巨噬细胞吞噬墨汁试验对天然免疫功能进行检测，淋巴细胞转化试验对细胞免疫功能进行检测，双向免疫扩散试验、酶联免疫吸附试验对体液免疫功能进行检测。 结果：细胞免疫的检测（淋转实验）结果全为阴性，吞噬细胞功能的检测（吞噬实验）结果为阴性，体液免疫的检测（双扩实验和ELISA）结果有三项测定项目为阳性。虽然本实验不足以证明CTX对免疫功能的抑制作用，但CTX的免疫抑制功能Objective: cyclophosphamide was applied to establish the mice immunosuppression model and detection whether the immunosuppression model success or not. Meyhod: Divid the 16 mice into four groups, each group of four. Divid the four mice into two groups, control group and immunosuppressive groups. On the first day giver primary immune to the two groups of fou mice, Control group mice were injected NS 0.4ml and OVA - AL (OH) 3 0.4 ml, Immunosuppressive mice were injected NS 0.4ml and OVA - AL (OH) 3 0.4 ml. In 8, 10, 12, 14 days inject 0.4 ml NS to the control group mice, inject 100 mg/kg CTX to the immunosuppressive mice. In the fifteenth day give strengthen immunity to the two groups of mice. Control group mice were injected NS 0.4 ml and OVA - AL (OH) 3 0.4 ml, Immunosuppressive mice were injected NS 0.4ml and OVA - AL (OH) 3 0.4ml,which means the four groups were NS-NS group, OVA-NS grpoup, NS-CTX group, OVA-CTX group.,Immunosuppression model is established. Use macrophage cell in abdominal cavity of mice phagocytosis ink experiment to test the natural immune function, The lymphocyte transformation test to test the cellular immune function, double immunodiffusion test, ELISA to test the humoral immune function. Result: The results cellular immune detection (lymphocyte transformation test) were all negative, the result Phagocytes function detection (Consuming experiment) was all negative, three of