郑大学报12邢华燕的1770羟基喜树碱3重点解读.doc

10-羟基喜树碱对类风湿关节炎患者Th17细胞的抑制作用 ? 王燕1），孟令杰2），王建平1）#，张俊峰3），刘乃嘉4） 郑州大学第五附属医院风湿免疫科 郑州 450052 2）郑州铁路职业技术学院医学技术系 郑州 450052 3）郑州大学第五附属医院检验科 郑州450052 4）郑州大学08级临床医学系临床医学五年制 郑州 450001 # 通讯作者:王建平，1956年12月生，博士，教授,主任医师，研究方向：免疫学，jianpingwang1208@126.com 河南省科技厅计划重点攻关项目 112102310610 关键词10-羟基喜树碱类风湿关节炎Th17细胞摘要 目的：探讨10-羟基喜树碱对类风湿关节炎（RA）患者Th17细胞功能的抑制作用。方法 选取60例RA患者随机分为三组(10-羟基喜树碱组、甲氨喋呤组及未给药的阴性对照组)，选取20例健康查体者为空白对照。采集各组患者外周血并分离单个核细胞进行培养，流式细胞术检测外周血Th17细胞表达的百分率，ELISA法检测培养液中IL-17水平。结果 RA患者Th17细胞阳性率显著高于正常对照。10-羟基喜树碱组和MTX组患者Th17细胞阳性率均明显下降，与未用药物干预组比较差异有显著性（P0.05），而两用药组间比较下降无显著性差异（P 0.05）。RA患者用药前PBMC培养上清中IL-17水平明显高于健康对照组，用药后患者IL-17水平显著低于用药前（P0.05）。两组间比较下降幅度无显著性差异（P 0.05）。结论 HCPT与MTX对RA患者Th17细胞的功能均具有抑制作用，提示HCPT有可能在RA治疗方面具有潜在价值。 The study of the HCPT in inhibiting the Th17 cells with RA 1)Fifth Affiliated Hospital of Zhengzhou University, Rheumatology Division, Zhengzhou 450052, China 2)Zhengzhou Railway Vocational and Technical College of Medical Technology, 08 Zhengzhou University, Zhengzhou 450052 3) Department of Clinical Laboratory, the Fifth Affiliated Hospital, Zhengzhou University, Zhengzhou, 450052, 4）China Department of Clinical Medicine Clinical five-year Zhengzhou 450001 Key words：TH17 cells; Rheumatoid arthritis; HCPT, IL17 Abstract：Objective To investigate the inhibition of Th17 cells in peripheral blood of patients with rheumatoid arthritis(RA) by HCPT. Methods Sixty active RA patients were enrolled in this study and divided into three groups(HCPT、MTX and control).Twenty persons are the health control. PBMC were separated and cultured. Intracelluar flow cytometirc detect the Th17 cells in peripheral blood. The level of IL-17 were tested by ELISA. Results The positive of the Th17 in RA was higher than that in health control. Both the HCPT and MTX can inhibited the function of Th17 cells.The level of IL17 in RA were higher than that in health control. HCPT and MTX can inhibited the lever of IL17. Conclusion HCPT can inhibited the function of TH17 cells an