莪术油口服吸收机理及制剂前研究9.docVIP

Chapter 3: transport mechanistic study of zedoary turmeric oil 3.1. Materials 3.1.1. chemicals Lucifer yellow was purchased from Sigma Chemical Co., USA. Propranolol was obtained from Synco. Acetonitrile and methanol for HPLC analysis were purchased from Merck (Darmstadt, Germany). Water for HPLC analysis was prepared using a Millipore Milli-Q? Plus system (Millipore, Bedford, MA, USA). Other chemicals and reagents used were at least of analytical grades. 3.1.2. materials for cell culture Caco-2 cells were obtained from the American Type Culture, No. HTB-37, Passage: 25-55 (Rockville, MD, US