血浆血清体液蛋白质组学(改进版柳满然)资料.ppt

* 常用串联质谱检索工具 SEQUEST: F/sequest PepFrag: P ProteinProspector: P Mascot: * ESI-MS/MS MAIDI-TOF 图像分析 确定差异点 差异蛋白质质谱分析 数据处理与生物信息学分析 数据搜寻蛋白质鉴定 条件A:样品 条件B:样品 基于2DE的差异蛋白质组分析技术流程 IEF:利用等电点差异分离Pr SDS:利用分子量差异分离Pr * Provides a hard-copy record of separation Allows facile quantitation Separation of up to 3000 different proteins Highly reproducible Gives info on Mw, pI and post-trans modifications Inexpensive Limited pI range (4-8) Proteins 150 kD not seen in 2D gels Difficult to see membrane proteins (30% of all proteins) Only detects high abundance proteins (top 30% typically) Time consuming 30% of spot