干细胞产品.说课.pptx

干细胞研究热点 ;干细胞研究文章 ;干细胞研究文章 ;干细胞研究文章 ;Stem Cell Science On the Rise in China;Stem Cell Science On the Rise in China;干细胞;Outline ;Differentiation of human tissues ;what are stem cells?;Classification of stem cells;Classification of stem cells;Types of stem cells;Embryonic stem cells;;Human embryonic stem cells;hESCs;James A. Thomson, et al. Science 282, 1145 (1998);Characterization of hESCs;Teratoma;;产品相关性 ：;Accutase dissociation to single cells maintains the proliferation rate of hESCs;Accutase-passaged hESCs maintain high levels of undifferentiated cells;A ROCK inhibitor Y-27632 permits survival of dissociated human embryonic stem cells;A ROCK inhibitor Y-27632 permits survival of dissociated human embryonic stem cells;A ROCK inhibitor Y-27632 permits survival of dissociated human embryonic stem cells;A ROCK inhibitor Y-27632 permits survival of dissociated human embryonic stem cells;So what’s the problem?;Before iPS;Somatic Cell Nuclear Transfer;SCNT;A Possible Solution…;Types of stem cells;Induced pluripotent stem cells (iPSCs);What is cellular reprogramming?;Yamanaka. (2007) Cell stem cell Vol 1, 39-49;Reprogramming Timeline;Advances in technology for iPS cell generation;;Non-integrative technology eliminates risk of insertional mutagenesis Stoichiometric control of individual reprogramming factors Eliminates need for backend screening of virus Faster iPS cell generation, colonies appear within 16 days Reproducible protocol using normal and diseased cells;相关产品 ：;What is the promise of hESC and iPSC research?;Inducing iPSCs using modified mRNA ;Bright-field images taken during the derivation of RNA-iPSCs (RiPS) from dH1f fibroblasts;Generation of RNA-Induced Pluripotent Stem Cells;Disease model ;Wow!;A Cure!;Types of stem cells;HSC (hematopoietic stem cells);;HSC;Hierarchy of hematopoietic stem cells;Hierarchy of hematopoietic stem cells;Differentiation of HSCs;Hierarchy of hematopoietic stem cells;Generation of function red blood cells and platelets form hema