烧烫伤创面感染的小鼠模型构建-中国实验动物学报.docVIP

周传农修改： 英文摘要 — 已修改 正文中的英文书写 — 已顺 图与表的英文 — 已顺 文献的英文书写 — 已顺 原文稿中，第1图缺图题，现已加上。后面的图的顺序编号也已经修改过了。 烧烫伤创面感染的小鼠模型构建 沈娟,2 金小宝2 丁静2 朱家勇2 （1. 广东药学院生命科学与生物制药学院 广州5100062. 广东药学院广东省生物活性药物研究重点实验室 广州510006） 摘要目的。方法15 s。伤后48 h，取创面组织进行HE染色观察，筛选最佳创面烫伤时间。另取72只小鼠制成深II度烫伤创面，采用擦刮法分别接种20 μL菌浓度为1×106、l×107、1×108 CFU/mL金黄色葡萄球菌标准菌株ATCC 25923的菌液。接种细菌72 h后接种细菌后72 h，取创面组织HE染色观察创面炎症反应情况，并测定3 d、7 d、14 d的皮肤菌负荷，筛选最佳的细菌接种浓度。最后，按最佳条件建模后，观察创面的完全愈合时间以及创面愈中、愈后的组织学变化，以确定此创面感染模型是否建立成功。结果 组织学结果表明，10 s为深II度创面的理想致伤时间，最佳接种菌浓为l×108 CFU/mL，此时期，14 d内菌负荷均高于l×105 CFU/g。该模型的创面愈合时间（21±0.95 d）较正常创面愈合时间（15.92±0.34 d）明显延长（P0.01），炎性反应明显，愈后不佳。结论 烧烫伤创面感染的小鼠模型构建成功，可作为感染创面防治研究的实验动物模型。 【关键词；；；Bal/c小鼠 中图分类号 Establishment of a mouse model of burn wound infection SHEN Juan1,2, JIN Xiao-bao2, Ding Jing2, ZHU Jia-yong2 ( 1. School of Life Science Biopharmacology, Guangdong Pharmaceutical University, Guangzhou 510006, China) (2. Guangdong Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou 510006) 【Abstract】 Objective To establish a simple mouse model of burn wound infection and provide useful tool for studies of prevention and treatment of burn wound infection. Method Self-made wood burn board and boiling water was used to generate burn injury (in a diameter of 8 mm) on both sides of the back in 30 Balb/C mice for different duration (5 s, 10 s, 15 s, with 10 mice in each group). 48 h post-burn, the wounds were examined by histology to determine the depth of injury. Deep II degree scald on the back in other 72 mice was generated. Immediately after burn，20 μL of bacteria suspension containing 1×106, l×107 or 1×108 CFU/mL Staphylococcus aureus ATCC 25923 was inoculated to the wounds by scraping, respectively (24 mice for each group). Inflammatory reaction of the wounds was examined with HE staining at 72 h post inoculation for screening the best bacteria inoculation concentration. On day 3, 7 and 14 post inoculation，the amount of wound bacterial load was assayed, respectively. Finally, the best condition was chosen