Cex1p is novel cytoplasmic component of the Saccharomyces ....docVIP

Cex1p is a novel cytoplasmic component of the Saccharomyces cerevisiae nuclear tRNA export machinery Andrew T. McGuire and Dev Mangroo Materials and methods Plasmid Construction The 2-(m pYX242 vector containing a LEU2 selection marker and pET19b were obtained from Novagen. The pRS416-CEN-URA3 vector was purchased from Stratagene. The plasmids pYX242-UTP8, yEPLAC195 carrying the synthetic yeast tRNATyram gene, pCEN-URA-GAL1 and pYX242-LOS1 were described before (Steiner-Mosonyi et al., 2003). pUN100 and pUN100-ARC1 were obtained for Dr. E. Hurt, University of Heidelberg, Germany (Simos et a