nature上有关茶的优秀论文.docx

EGCG regulates cell growth, cell cycle and phosphorylated nuclear factor-Kb in human dermal fibroblasts EGCG对人体皮肤成纤维细胞的生长、细胞周期和磷酸化的核因子的影响 Aim: to investigate the effects of EGCG, the main polyphenol in green tea, on cell growth, cell cycle and phosphorylated nuclear factor-kB expression in neonatal human dermal fibroblast. Methods: The proliferation(增殖) and cell-cycle of nHDFs were determined using WST-8 cell growth assay(测定) and flow cytometry(流体细胞仪), respectively(分别地). The apoptosis(细胞凋亡) was examined using DNA ladder and Annexin V-FITC assays. The expression levels of pNF-κB and cell cycle-related genes and proteins in nHDFs were measured using cDNA microarray(芯片) analyses and Western blot. The cellular uptake(摄取) of EGCG was examined using fluorescence (FITC)-labeled EGCG (FITC-EGCG) in combination with confocal microscopy(多聚焦显微镜). Results: The effect of EGCG on the growth of nHDFs depended on the concentration(浓度) tested. At a low concentration (200 μmol/L), EGCG resulted in a slight decrease in the proportion(比例) of cells in the S and G2/M phases of cell cycle with a concomitant(附随的) increase in the proportion of cells in G0/G1 phase. At the higher doses(剂量) (400 and 800 μmol/L), apoptosis(细胞凋亡) was induced. The regulation(调节) of EGCG on the expression of pNF-κB was also concentration-dependent(浓度依赖性), whereas(但是) it did not affect the unphosphorylated(磷酸化) NF-κB expression. cDNA microarray(芯片) analysis showed that cell cycle-related genes were down-regulated by EGCG (200 μmol/L). The expression of cyclins(细胞周期蛋白) A/B and cyclin-dependent kinase(致活酶) 1 was reversibly(可逆地) regulated by EGCG (200 μmol/L). FITC-EGCG was found to be internalized(内化) into the cytoplasm(细胞质) and translocated(易位) into the nucleus of nHDFs. Conclusion: EGCG, through uptake(摄取) into cytoplasm(细胞质), reversibly(可逆地) regulated the cell growth and expression of cell cycle-related proteins and genes in normal fibroblasts. Introduction Food bioactives(生物活性物质) with strong antioxidant(抗氧化剂) pr