生物分离工程-双语版 4.ppt

Chapter 4  interfacial protein adsorption and inactivation I introduction Protein adsorption phenomena examples (1) clinical implants (2) mammalian and bacterial cell adhesion to surfaces (3) initiation of blood coagulation (4) solid-phase immunoassay (5) protein binding to cell surfaces Protein-enzyme interfacial interaction in bioseparation (1) gas-liquid (2) liquid-liquid (3) liquid-solid I introduction Protein interfacial adsorption Low protein concentration (no barrier ) High surface protein concentration (barrier) A electrostatic B steric C osmotic effect Proteins adsorption on Gas-liquid interface (1) enzyme adsorbed unfolded partially active or inactive state as rigid film (2) proteins unfolding depends on conformational stability (3) interfaces are primarily responsible for protein inactivation (4) Shear associated damage is severe when gas-liquid interface present A pump B centrifuges C ultrafiltration (5) proteins on adsorption at fluid interfaces undergo a change from their globular configuration to an extended chain structure (6) change of protein structure lead to activity changes Proteins adsorption 1 protein molecules in aqueous solution have a hydrophobic interior and a hydrophilic exterior 2 structural perturbation-rearrangements involving changes in interatomic distance affect the intermolecular van der waals interaction 3 charge groups in the apolar interior of the protein molecule tend to form ion pairs. 4 intramolecular peptide hydrogen bonds are more favorable than peptide-water interaction 5 the variations in the hydrogen bonding, ion pairing, hydrophobic interaction affect the rotational motion Proteins adsorption 6 Increase of entropy originating from the dehydration of the interface-surface, and dehydration of structural changes are the driving force. 7 pH and shaking significantly affect the rate of transport of protein to interface. 8 Surfactants occu