扩增阻滞突变系统法检测非小细胞肺癌患者外周血EGFR突变研究.pdfVIP

ABSTRACT Objective： To the ofthe of bloodalternativesof peripheral explorefeasibilityapplication tumortissueto detect factor in EGFR(epidermalgrowth receptor)mutation smallcell mutation refractory NSCLC(non lungcancer)byARMS(amplification system)． Methods： of68 from I collecttumortissuesand venousblood pairedperipheral patients thoracic ofthefirstaffiliated of nanchanguniversity，allpatients surgery hospital wereextractedtheDNAQIAGEN treatment，thesesamples by acceptedsurgical DNAextractionthendetectedEGFRmutationthe human kit，and gene by AmoyDx 21 EGFRmutationdetection the betweenEGFR gene kits，weanalyzedrelationship in bloodandtumortissueofNSCLC mutationandclinicalfeature gene peripheral in to the ofEGFR mutations consigency gene patients，furthermorecompare ．and the bloodandtumor test analyzed peripheral tissue．byusingchi·square andclinicalfeature． betweendifferentmutation relationship type results： 1、EGFRmutationsoftumortissueand bloodwas i