酶偶联法催化还原潜手性酮类化合物研究.pdf

酶偶联法催化还原潜手性酮类化合物研究.pdf

基)乙醇 ABSTRACT reduct弱e弱ato chiml tlle of tllealdo.keto D咖gprocesstaking catal)rstsyntllesizecompoundS, me will attlles锄etime.These aremore man beconsumed expeIlsivepr印ared co锄zymes coeI]【zymes s切bilitiesofmese itisdi伍culttoreusemese products.Meanwhile,tllecoeI】目恤鹤arepoor,a11d isleSs tocasta of coeIlzyTnes.h1iIldus缸yproduction,itlikely large锄ount cons臼mctionofme emcientcofactor andtlle useof areme System recycliIlgcoenzymes higllly regeneration mustbesolVediIltlle mat present. probl锄s wasusedin hlordertosolVethe cofactor tecllIlique probl锄of regeneration,geneengiIle甜ng this me for chain paper.Withgenomesof£D妣rD,妒西ast锄platep01),IIlerase w汕thec枷er dehydrogenase(FDH)wasobtained.BycoIlIlecting pET28a, 锄plification,fomate recombinant wasobtained.Tllent11e wastmsf0珊edintoEcD,fBL21 plasmidpFDH-pET28a plasmid and 1一FDH.IIl“s innuencesoftlle concen昀tion obtainEcD,fBL2 (DE3)to paper,me t锄perature,the metimeonthesoluble werestlldied.The FDHwasdetenllined. proteinexpression enzymeactiVi够of kinds acid Two ofphamace

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