神经生物学-绪论预案.ppt

Morphine-induced CPP Linear dose effect relationship -2 -1 0 1 0 100 200 n 9-12 Lg of Mor Dose mg/kg difference s EA Suppression of CPP for Morphine Control Restrain Foot shock Needle 100Hz 2Hz 2/100Hz 0.0 2.5 5.0 7.5 10.0 *** *** Time Spent in the Drug-pairing Side min n 18~20 Effect of EA of Different Frequencies Effect of EA Reversed by Naloxone n 8~9 Needle 2/100Hz 2Hz 0.0 2.5 5.0 7.5 10.0 *** *** 2/100Hz +NX 5mg/kg 2Hz +NX 1mg/kg Reinstatement of morphine’s CPP on rats by stress Time Spent in the Drug-pairing Side min Fading of Morphine’s CPP with Time Time from Last Drug-pairing days 0.0 2.5 5.0 7.5 10.0 1 3 5 7 *** *** * Foot Shock 2 Hz 2 Hz + 0.0 2.5 5.0 7.5 10.0 NX 1mg/kg n 12 Relapse Induced by Intermittent Footshock 9 days after last drug-pairing Saline Morphine 4mg/kg n 12 *** Or drug priming 100 Hz 应激 引起 复吸 条件反射随时间而消退 Days of observation Suppression of heroin craving by HANS in humans 人体心瘾观察及HANS抗心瘾作用 Drug use over 1 year aver 4.6 y ; Detoxification over 1 month; Male, Aver 25 y; n 29-30 Craving score VAS 30 min per day Pre-HANS HANS-treating Post-HANS 10 20 30 100 Hz 2 Hz Mock 2/100 Hz 0 0 1 2 3 4 5 No relapse over 1 year Clinical Base Method of Detox. HANS for Rehab Success over 1 year Success Rate Hainan HANS Yes 11 / 56 Ca 20 % Shanghai HANS and others Yes 9 / 100 Ca 10% Canton HANS Not constant 9-20 / 500 Ca 2-4 % No relapse over 1 year 一年不复吸 Aim: HANS detox. + HANS ready in the pocket 30＋% success over 1 year Opiate craving Suppression of drug craving by HANS Cocaine craving 2 Hz 100 Hz 100 Hz 2 Hz Electroacupuncture treatment for drug addiction VTA Opiate GABA NAc DA DAT Cocaine Euphoria Enk Dyn Electroacupuncture 2 Hz 100 Hz Presynaptic inhibition Substitution μ κ μ μ A 100 bp 2000 bp 1000 bp 750 bp 500 bp 250 bp DL 2000 NS CPP 2 Hz 100 Hz restrain GAPDH 532 bp PPD 250 bp Effect of 100Hz EA on the level of PPD mRNA in NAc of CPP rats B 0.0 0.5 1.0 1.5 Grey scale ratios PPD/GAPDH Treatment * # n 4