4GeneticScreenC分析.pptx

遗 传 分 析;IV. 分析突变;IV. 分析突变;突变对应了多少个基因？;“饱和”遗传筛选;2号染色体突变致死基因数字估算;有什么没发现？;果蝇胚胎发育;Hedgehog (Hh) Regulates Drosophila Limb Development;Sonic Hedgehog (SHh) Regulates Limb Development in Vertebrates;Hh Regulates Wing Pattern in Drosophila;SHh Regulates the Digit Pattern of Vertebrate Limbs;II. 选择研究模型：黑腹果蝇;进行遗传筛选的几点考虑;;遗传学研究;一些著名的遗传筛选;Heberlein的醉酒仪;进行遗传筛选的几点考虑;各种模式生物;“We should like to attack the problem of cellular development in a similar fashion, choosing the simplest possible differentiated organism and subjecting it to the analytical methods of microbial genetics. … Thus we want a multicellular organism which has a short life cycle, can be easily cultivated, and is small enough to be handled in large numbers, like a micro-organism. It should have relatively few cells, so that exhaustive studies of lineage and patterns can be made, and should be amenable to genetic analysis.” Sydney Brenner, 1963;理想的遗传学模式生物;现实的遗传学模式生物;常用遗传学模式生物的特性;用最简单的模型工作;进行遗传筛选的几点考虑;如何设计筛选流程;酵母单倍体筛选;;