圆二色谱原理与应用.pptVIP

CD特点 测量的θ非常小 CD测量的为2.32*(AL-AR) 弧度 以样品有圆二信号一定要有紫外吸收但有紫外吸收不一定由远而信号 CD谱在蛋白质研究中的应用 Recommended Methods For determination of globular protein conformation in solution: SELCON, CDNN and K2D. For determination of polypeptide conformation: LINCOMB with a suitable polypeptide set of references. For determining the effects of mutation s, ligands and perturbants on protein structure: LINCOMB. For evaluating the number of folding states giving rise to a set of spectra: The CCA algorithm and SVD. 从CD谱分析准确性 对全?、 ? /?和变性蛋白质的准确度90-100% 对? + ?的准确度为85% 对全?的准确度为75% Applications of CD in structural biology De