生物信息学 实验五、多重序列比对和分子系统发育分析研讨.docVIP

实验五、多重序列比对和分子系统发育分析 1、用ClustalW等软件进行序列比对 先用blastn找出多组相似序列 [2] Multiple sequence alignment [1] Confirm that all sequences are homologous [2] Adjust gap creation and extension penalties as needed to optimize the alignment [3] Restrict phylogenetic analysis to regions of the multiple sequence alignment for which data are available for all taxa (delete columns having incomplete data). [4] Many experts recommend that you delete any column of an alignment that contains gaps (even if the gap occurs in only one taxon) Figure. Evolutionary relationships of 33 taxa (lineariz