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受体激酶SLA1在叶倾角调控过程中的功能研究   摘 要 水稻重要的农作物，具有良好是提高产量的有效手段之一，我室前期在对水稻中一些类受体激酶的功能进行研究时，发现其中一个类受体激酶通过RNA干涉后植株具有叶片直立、叶倾角变小的表型，而生长发育的其他方面未我们将此类受体激酶命名为OsSLA1(Oryza sativa Small Leaf Angle 1)。 本文对OsSLA1功能进行了进一步探究，主要包括以下几个方面：(1)OsSLA1OsSLA1 RNAi转基因植株OsSLA1 RNAi转基因植株(2) 在检测不同激素处理对叶倾角的影响时发现，OsSLA1 RNAi转基因植株对BL不敏感有趣的是，当用BLIAA共同处理时，OsSLA1 RNAi转基因植株对BL的不敏感性得到了恢复(3) qRT-PCR检测BR及IAA通路相关基因表达OsSLA1 RNAi转基因植株，OsSLA1可能同时参与BR和IAA两个途径调控叶倾角。 (4) 酵母双杂交实验显示Os(K) -AD和OsSLA1(K)-BD之间不存在相互作用(5) OsSLA1亚细胞显示其在质膜及质膜周围区域表达呈不连续状态，可能定位在一些囊泡结构中这些研究结果对于OsSLA1参与叶枕调控提供了进一步的实验证据。 关键词： 水稻叶倾角OsSLA1 BR IAA Abstract Rice as one of most important crops, a good form of plant can improve its yield. Therefore, the study of regulatory mechanism on rice plant type is very important both in theory and practice. After our previous studies on some receptor-like kinase genes, we found that one receptor-like kinase gene interfered by RNAi leaded to leaf angle smaller but had no effects on other aspects of plant growth. We named this receptor-like kinase gene OsSLA1 (Oryza sativa Small Leaf Angle1). In this paper, the functions of OsSLA1 were further explored, and the results are described as follows. (1) The OsSLA1 RNAi transgenic plant had smaller leaf angle and erect leaves, but its height, seed setting rate and thousand seed weight were not changed. Observing the cells of pulvinus, we found that the adaxial cells getting smaller and the vascular bundle getting bigger, which meant the cells of pulvinus lead to the features of leaf inclination smaller. (2) The effects of different hormones treatment on leaf angle showed that OsSLA1 RNAi transgenic plants were insensitive to BL treatment, slightly insensitive to IAA, but no obvious sensitivity difference to GA, KT as well as ABA compared with WT. It is interesting to notice that the insensitivity to BL in the OsSLA1 RNAi transgenic plants seemed to be restored when treated both with BL and IAA. (3) qRT-PCR assay showed that the expression