基因工程复习资料(初步整理版).docVIP

基因工程题型 名词解释（英文） 填空题 单项选择题 简答题 应用分析题 一、名词解释 1．Blotting（影迹）：Blotting is the process of identiying specific bands of intrerest amongst all the bands bound to the nitrocellulose. Done with specific probes or antibodies. 2．Probe：Probe is a specific DNA or RNA fragment which can bind with the sample DNA or RNA for dection. 3．Cosmid：Plasmids have been constructed which contain a fragment of λDNA including the cos site. These plasmids have been termed cosmids and can be used as gene-cloning vectors in conjunction with the in vitro packing system. 4．SD Sequence：The special DNA sequence which binds to 3`end of 16S rRNA and initiates synthesis of protein. 5. Inclusion body （包涵体）: “Inclusion bodies typically refers to aggregates of protein in bacterial cytoplasm. Inclusion bodies are classically thought to be comprised of misfolded protein. 6. Palindromes（回文序列）：A segment of double-stranded DNA in which the nucleotide sequence of one strand reads in reverse order to that of the complementary strand. . Incompatibility of plasmids （不相容质粒）：Plasmid incompatibility is the inability of two different plasmids to coexist in the same cell in the absence of selection pressure. . Consensus sequence（一致序列）：If the transcription star point is assigned the position +1 then this consensus sequence consists of the -35 region(TTGACA) and the -10 region, or Pribnow box(TATAAT).RNA polymerase must bind to both sequences to initiate transcription. . cDNA Library：A cDNA library is the set of all the mRNAs contained within a cell. Referred to as cDNA these reverse transcribed mRNAs are collectively known as the library. 1． 因研究重组DNA技术而获得诺贝尔奖的科学家是( ) (a)A．Kornberg (b)W．Gilbert (c)P．Berg (d)B．McClintock 2． 第一个作为重组DNA载体的质粒是( ) (a)pBR322 (b)ColEl (c)pSCl01 (d)pUCl8 3． 关于宿主控制的限制修饰现象的本质，下列描述中只有( )不太恰当。 (a)由作用于同一DNA序列的两种酶构成 (b)这一系统中的核酸酶都是Ⅱ类限制性内切核酸酶 (c)这一系统中的修饰酶主要是通过甲基化作用对DNA进行修饰 (d)不同的宿主系统具有不同的限制-修饰系统 4．Ⅱ型限制性内切核酸酶： ( ) (a)有内切