真核生物转录机理与调控探索.ppt

Actually, the activity of the different polymerases was determined using α-amanitin. Alpha-amanitin is produced by a kind of mushroom and is deadly poisonous to human beings. 实际上，不同聚合酶的活性是使用了一种叫做α鹅膏蕈碱的毒素来测定的，这种毒素是一种蘑菇产生的，对人的毒性非常大。 In order to see which genes are transcribed by which polymerase, cells are exposed to α-amanitin. It was found that, under very high concentrations of α-amanitin, only rRNA precursor is produced. This means that rRNA precursor is produced by RNA polymerase I. 为了弄清哪些基因由哪种聚合酶转录，细胞被暴露在α鹅膏蕈碱中。暴露在很高浓度α鹅膏蕈碱的细胞只产生rRNA前体，意味着这正是RNA聚合酶I所转录的基因。 Then, cells expose to low co