HPLC-DAD结合SWATLD二阶校正方法快速测定.doc

HPLC-DAD结合SWATLD二阶校正方法快速测定 丹参和复方丹参片中三种丹参酮含量 张喜华，吴海龙*，张树荣，王予，俞汝勤 长沙410082 湖南大学 化学生物传感与计量学国家重点实验室；*Email: hlwu@hnu.edu.cn 将高效液相色谱-二极管阵列检测 HPLC-DAD 与化学计量学基于自加权交替三线性分解 SWATLD 算法的二阶校正方法相结合，直接快速测定了种实际复杂体系丹参药材和复方丹参片中隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA含量。本实验方法：色谱柱：WondaSil C18（150 mm×4.0 mm，5.0 μm）；流动相：乙腈 – 0.1 %甲酸水溶液（95:5 v/v）；流速：1.0 mL /min；检测波长范围：190~400 nm；柱温：35 ℃。用SWATLD得到丹参和复方丹参片中隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA的平均含量分别为 0.472±0.052 mg/g、 0.421±0.059 mg/g、 0.884±0.022 mg/g和 0.953±0.097 mg/g、 0.466±0.039 mg/g、 1.119±0.021 mg/g，分别计算了它们的加标回收率。另外，采用高效液相色谱-质谱技术获得该两种体系中的三种丹参酮定量分析结果与实验预测结果。结果表明，该方法能够实现未知复杂背景干扰共存下三种丹参酮含量的直接快速定量测定。 隐丹参酮丹参酮Ⅰ丹参酮ⅡA自加权交替三线性分解 参考文献 [1] Chen, Z. P.; Wu, H. L.; Jiang, J. H.; Li, Y.; Yu, R. Q. Chemometrics and Intelligent Laboratory Systems. 2000, 52 1 : 75-86. [2] Li, Y. N.; Wu, H. L.; Qing, X. D.; Li, Q.;Fu, H. Y.; Yu, Y. J.; Yu, R. Q. Ana. Chim. Acta. 2010, 678 1 : 26-33. Simultaneous Determination of Three Tanshinones in Radix Salviae Miltiorrhizae and Fufang Danshen Tablet Using HPLC-DAD Coupled with Self-weighed Alternating Trilinear Decomposition Method Xi-Hua Zhang, Hai-Long Wu*, Shu-Rong Zhang, Yu Wang, Ru-Qin Yu State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082; Email: hlwu@hnu.edu.cn A novel second-order calibration method has been developed for simultaneous determination of cryptotanshinone , tanshinone Ⅰand tanshinone ⅡA in Radix Salviae Miltiorrhizae and Fufang Danshen tablet samples by using HPLC-DAD coupled with the self-weighed alternating trilinear decomposition SWATLD algorithm. The HPLC assay was performed on WondaSil C18 column with constant gradient elution of acetonitrile and 0.1% phosphoric acid solution（95:5， v/v） at a flowrate of 1.0 mL /min. The column temperature was set at 35 ℃. The range of detection wavelength was 190.0~400.0 nm. The quantitative prediction of cryptotanshinone, tanshinone Ⅰ and tanshinone ⅡA in aforementioned samples were 0.472±0.052 mg/g, 0.