汞对伊乐藻、菹草生理生化特性影响以及外源钙缓解作用.pdfVIP

摘要 源多胺含量的改变抑制了植物体内ROS的积累、膜脂过氧化、光合色素及抗氧化物 质的分解，同时有效促进可溶性蛋白合成，提高了抗氧化酶活性和抗氧化物质含量， 使菹草无菌苗维持较高的总抗氧化能力，并通过调节脯氨酸代谢来减轻H92+胁迫对 菹草的伤害，增强植物对重金属H92+的耐受能力。 关键词：汞， 钙，氧化胁迫，沉水植物 Il Abstract Abstract Sterile seedlings of Potamogeton crispus L．were cultured by tissue culture technique， and were used aS experimental materials．In this study,the effects of exogenous calcium (Ca)application on mercury(Hg)accumulation，mineral nutrition，photosynthetic pigments，superoxide anion(02‘’)，hydrogen peroxide 0-1202)，malondialdehyde(MDA)， vitamin C and E，glutathione(GSH)and polyamines metabolism of the tested plants under the stress of 1．5小Hg”stress wele investigated．The results demonstrated that，(1)Hg’ stress induced a substantial accumulation of H92+at 1 65．79 gg／g FW and resulted in serious imbalance of nutrient elements，such嬲inhibiting the absorption of P，K，Fe，Mg and Na， whereas exogenous Ca2+significantly reduced the accumulation of H92+by 66．4％and effectively reduced the Hg·induced imbalance of nutrient elements in Potamogeton crispus L．．(2)H92+treatment severely reduced chlorophyll concentration，chlorophyll a／b ratio， and raised 02。generation rate，H202 and MDA contents，while increased the levels of Vc， VE and GSH．Application of exogenous Caz+markedly retarded the degradation of photosynthetic pigments and also significantly improved the levels of antioxidant capacity in the tested plants．(3)Hg”treatment enhanced polyamine oxidase(PAO)，diamine oxidase(DAO)，and arginine decarboxylase(ADC)activities，moreover reduced ornithine decarboxylase(ODC)result in decrease of putrescine(Put)and spermine(Spin)and slightly increase of spermidine(Spd)content．Exogenous Ca”altered polyamines content of H92’stress by modulating polyamines metabolizing enzymes activities，mainly presented as significant increase in Put and Spm contents，and further increased the ratio of free(Spd+Spm)／Put．These results indicated that the supplement of exogenous Ca‘+ relieved the phytotoxicity of H92+to Potamogeton Crispus L．by dep