Chapter 3：.pptVIP

In the external standard method, the solute chosen as the reference is chromatographed separately from the sample. However, results from two chromatograms will be compared so chromatographic conditions must be maintained extremely constant. To reduce the effect of any changes in the operating conditions the sample and reference solutions can be chromatographed alternately. The data from the reference chromatograms run before and after the sample are then used for calculating results of each assay.With the external standard method, the reference standard (or standards) can be chosen to be the