INTRO TO TFF 切向流过滤简介.docVIP

Scientific Technical Report PN 33213 Introduction to Tangential Flow Filtration for Laboratory and Process Development Applications Larry Schwartz, Senior Technical Manager, Pall Life Sciences Kevin Seeley, Ph.D., Scientific and Laboratory Services Introduction Tangential flow filtration (TFF) is a rapid and efficient method for separation and purification of biomolecules. It can be applied to a wide range of biological fields such as immunology, protein chemistry, molecular biology, biochemistry, and microbiology. TFF can be used to concentrate and desalt sample solutions ranging in volume from 10 mL to thousands of liters. It can be used to fractionate large from small biomolecules, harvest cell suspensions, and clarify fermentation broths and cell lysates. This report describes the basic principles that govern TFF and the use of TFF capsules and cassettes in laboratory and process development applications. Why Use Tangential Flow Filtration? 1) TFF is easy to set up and use – Simply connect the TFF device to a pump and pressure gauge(s) with tubing and a few fittings, add your sample to the reservoir and you’re ready to go. An example set-up is shown in Figure 1. 2) TFF is fast and efficient – It is easier to set up and much faster than dialysis. You can achieve higher concentrations in less time than with centrifugal devices or stirred cells. 3) Perform two steps with one system – You can concentrate and diafilter a sample on the same system, saving time and avoiding product loss. 4) TFF can be scaled-up or scaled-down. – Materials of construction and cassette path length allow conditions established during pilot-scale trials to be applied to process- scale applications. TFF devices are available that can process sample volumes as small as 10mL or as large as thousands of liters. 5) TFF is economical – TFF devices and cassettes can be cleaned and reused, or disposed of after single use. A simple integrity test can be performe