马昌杯第16章RNA的生物合成2014-11-26解读.ppt

* * He discovered RNA polymerase. * * * 7SL RNA is small cytoplasmic RNA(scRNA) Amanitin: 鹅膏蕈碱 Pol II is most sensitive to ?-amanitin, an octapeptide from Amanita phalloides (destroying angel mushroom) * All eukaryotic RNA polymerases typically have two large subunits and 12-15 smaller subunits. All are big, multimeric proteins (500-700 kD) All have 2 large subunits with sequences similar to ? and ? in E.coli RNA polymerase, so catalytic site may be conserved RNA polymerase II is central to eukaryotic gene expression于and has been studied extensively. Although this polymerase is strikin