血清肌酸激酶及其同工酶活性的影响(精选).docVIP

黄芪注射液对两种力竭运动大鼠血清肌酸激酶及其同工酶活性的影响 李永峰 韩勇 郭勇力 王传军 李晔 山东体育学院日照校区 山东日照 276826 摘要：目的：探讨黄芪注射液对两种力竭运动大鼠血清CK及其同工酶的影响，为减轻运动性肌肉损伤寻找有效的方法。方法：将50只大鼠随机均分为五组：安静对照组(n=10)、一次性力竭运动组(n=10)、多次力竭运动组(n=10)、服药+一次性力竭运动组(n=10)、服药+多次力竭运动组(n=10)。经过4周的实验后，测血清CK及其同工酶的含量。结果：一次力竭和多次力竭大鼠血清CK及其同工酶活性显著增高（P0.01），黄芪注射液对一次力竭和多次力竭大鼠血清CK及其同工酶活性有降低作用（P0.01）。结论：黄芪注射液对减轻两种力竭运动引起的肌肉损伤均有很好的作用。 关键词：黄芪注射液；力竭运动；肌酸激酶；同工酶 Effect of Radix Astragali injection on creatine kinase(CK) and isoenzyme of creatine kinase(CK-MM) level in rat serum after two kind of Exhausting Exercises Li Yongfeng，Han Yong，Guo Yongli，et al. Shandong Sports university，jinan，China 250014 Abstract: Objective: In order to search potent means for exercise-induced muscle damage, effect of Radix Astragali injection on serum creatine kinase(CK) and isoenzyme of creatine kinase(CK-MM) level in rats after two kind of Exhausting Exercises was observed. Methods: 50 Wister Rats were randomly divided into 5 group: normal control group(n=10), once exhausting exercise group(n=10), multiple exhausting exercises group(n=10), Radix Astragali injection filled the stomach and once exhausting exercise group(n=10) , Radix Astragali injection filled the stomach and multiple exhausting exercises group (n=10). After 4 weeks test, serum CK and CK-MM were tested Results: Afer exhausting exercise , CK and CK-MM in blood serum as well as were higher than that in normal control group（P0.01）.the change was prevented by Radix Astragali injection（P0.01）. Conclusion: Radix Astragali injection may help reduce exercise-induced muscle damage afer two kind of exhausting exercises. Key words: Radix Astragali injection； Exhausting Exercise； CKCK-MM 运动性肌肉损伤（exercise-induced muscle damage，EIMD）是一种高强度或/和长时间运动后发生的骨骼肌纤维的微细损伤，常表现为延迟性肌肉酸痛（delayed onset muscle soreness，DOMS），且伴有血液酶学指标的变化。消除运动性肌肉损伤是目前运动医学领域的重要课题。中药黄芪是临床常用的补气药，是一种有效的自由基消除剂[1]。研究表明[2]，运动引起的血清肌酸激酶（CK）及其同工酶的变化与自由基的大量生成有关。本研究旨在观察黄芪注射液对一次力竭和多次力竭运动大鼠血清CK及其同工酶的影响，以探求减轻运动性肌肉损伤、提高运动能力的有效方法。 材料与方法 1．1 实验动物及分组 实验用雄性SD大鼠50只，