pdgf-cc在静脉血栓溶解过程中的表达及其作用-第三军医大学学报.docVIP

血小板衍生生长因子CC在静脉血栓溶解过程中的表达及其作用(400010重庆，重庆医科大学附属第二医院血管外科) 【摘要】 目的:探讨静脉血栓溶解过程中血小板衍生生长因子CC（PDGF-CC）的表达，及其在静脉血栓溶解过程中的作用，为静脉血栓的临床治疗提供新的理论基础。方法: 使用成年雄性SD大鼠，建立大鼠下腔静脉血栓模型，分别在血栓形成之后1、3、7、14、21、28天收集下腔静脉壁及血栓标本（n=12）。应用Western blot技术及实时荧光定量PCR技术分别检测血栓自然溶解过程中大鼠下腔静脉及血栓内PDGF-CC蛋白表达变化及PDGF-CC mRNA的表达，取术后7天的大鼠下腔静脉及血栓标本，用于病理组织形态观察及PDGF-CC免疫组化检查。结果: Western blot检测大鼠下腔静脉血栓中PDGF-CC蛋白表达结果显示，术后1d到术后28天均有表达，以术后7d表达最为明显（P＜0.05）；Real-time qPCR技术检测大鼠下腔静脉血栓中PDGF-CC目的基因的结果为术后1d到术后28天均有表达（P＜0.05），术后7d相对表达量最高，为正常组的77.71倍；免疫组化显示术后7d的血栓标本中，新生血管周围有PDGF-CC的特异性表达。结论: PDGF-CC在大鼠下腔静脉血栓自然溶解过程中有的表达，并且在血栓中新生血管周围有特异性表达，这表明在血栓的溶解过程中，PDGF-CC可能促进静脉血栓的机化与再通。 【关键词】深静脉血栓; PDGF-CC; 血管新生 【中图法分类号】R654.4；R459.9 【文献标志码】 A Platelet-Derived Growth Factor CC is found in resolving venous thrombi Fu Jian,Luo Wenjun (Department ofVascular Surgery,the SecondAfiliated Hospital,ChongqingMedical University ,chongqing,400010,china) 【Abstract】Objective: The aim of this study was to determine the temporal changes and location of platelet-derived growth factor CC (PDGF-C) expression, which is a new member of the PDGF family, in a rat model of venous thrombus resolution.Design and Methods: Thrombi were induced in the inferior venae cavae of Sprague-Dawley rats by creating a stenosis to reduce blood flow by 80% to 90%,and damaging the inferior venae cavae endothelium. Thrombi, adjacent inferior vena cava wall were collected at 1, 3, 7, 14, 21, and 28 days after thrombus generation (n = 12). Reverse-transcription polymerase chain reaction and Western blot were performed to detect PDGF-CC expression. Tissues from three animals that were humanely killed 7 days after thrombus formation were prepared for histological examination. Immunohistochemistry was performed by the use of antibodies against PDGF-CC.Results: Laminated thrombi were reliably produced in thrombus animals. From 1 to 28days ,PDGF-C protein were expressed and increased until day 28days,7days is the highest .From 1 to 7days ,PDGF-C messenger RNA were expressed and increas