定量核磁共振实验基本知识.docVIP

定量NMR 实验 实验考虑： Good signal to noise 好的信燥比 90° pulse sufficiently short to excite the entire region uniformly Delay between pulses of at least 5 T1’s 脉冲延迟至少T1的5倍 Flat base line to start with and baseline correction 基线平坦和基线校正 Filter bandwidth set to at least twice the sw Enough data points to give 15 to 20 points per peak - remember that you lose half the points to the imaginaries in Fourier transformation Acquire at least 32k and zero fill several times – memory is cheap Exception to 32k for other nuclei requiring 1H decoupling - it is important to limit the acquisition time to as short as