第8章RNA转录后的加工分析.ppt

和传统酶的区别： (1)一般的酶是纯的蛋白质，而核酶是RNA或带有蛋白的RNA； (2)核酶既是催化剂又是底物。而酶仅催化反应。 The first step in mRNA 3 end formation is the selection of the site within the pre-mRNA for processing.? Cleavage polyadenylation specificity factor (CPSF), a multi-subunit complex, binds at the AAUAAA polyadenylation signal found 10-30 bases upstream from the 3 end of most mRNAs, while CstF binds to a UG-rich sequence typically found 10-30 bases downstream from the 3 end.? The co-operative binding of these two complexes to the RNA and protein-protein interactions between them sets up the subsequent cleavage and polyadenylation r