Beacon Designer Primer Express.pptVIP

4.Launch search by clicking the Search. Note: 1.Recommend the use of default values for TaqMan? search 2.The most important point to note is that the optimal primers are designed as close as possible to the TaqMan? probes without overlapping it. 3.If the sequence has selected SNP, Beacon Designer will design both wild and mutant TaqMan? probes for the sequence. 2.TaqMan? Probe Search Results 3.Predesigned TaqMan ? Probes Assay Ⅲ .Differences of two pieces of software There is an pdf of Beacon Designer vs Primer Express. It comes from PREMIER Biosoft Company Ⅳ .Supplement What is MGB LNA? MGB is the abbreviation of Minor Groove Binder. TaqMan probes conjugated with minor groove binder ligands (MGB) at the 3’-end hybridise to single-stranded targets with increased sequence-speci?city compared to ordinary DNA probes, allowing shorter probes to be used for hybridisation-based assays. In comparison with unmodi?ed DNA, MGB probes have higher melting temperatures (Tm). LNA is abbreviation of Locked Nucleic Acid It is a class of nucleic acids containing nucleosides whose major distinguishing characteristic is the presence of a methylene bridge that connects the 2’-oxygen of ribose with the 4’-carbon of theribose ring. (是一种核酸类似物，和普通核酸分子区别在于在其碳环的2氧原子和4碳原子位置引入亚甲基桥形成锁状结构，因此也称锁核酸。) LNA nucleosides allow increased stability of the nucleic acid duplexes formed between LNAs and other nucleic acids Usually, LNA/DNA duplexes have increased thermal stability (3–8 ℃ per modi?ed base in the oligonucleotide) compared with similar duplexes formed by DNA alone. Two pieces of software of the primer and probe design for Quantification and Allelic Discrimination assays ――Primer Express 3.0 Beacon Designer 7.0 Content Ⅰ. Brief introduction of the Primer Express 3.0 Ⅱ. Summary of the Beacon Designer 7.0 Ⅲ. Differences of these two software Ⅳ. Supplement Ⅰ. Brief introduction of the Primer Express 3.0 1. Designing Primers and Probes for Quantification Assays (No description)