分子生物学第五章资料.pptVIP

Now, how does capping take place? Actually, three enzymes are involved in the capping process. First, the RNA triphosphatase removes one phosphate from the 5’ end of the pre-mRNA. Second, guanylyl transferase adds a guanine nucleotide to make 5’-5’ linkage. Finally, methyl transferase adds a methyl group to the guanine nucleotide. 那么，加帽是如何进行的呢？实际上，加帽过程需要有三种酶参与。首先，RNA三磷酸酶首先从前体mRNA的5’端移去一个磷酸；之后鸟苷酸转移酶加上一个鸟嘌呤核苷酸以形成5’-5’键；最后，甲基转移酶在鸟嘌呤核苷酸上加上一个甲基。 These three enzymes stay together around the C-terminal domain of the Rpb1 subunit of RNA polymerase II. They act together to complete the capping pr