DNAExtraction-MichiganStateUniversity.pptVIP

DNA Extraction Outline Purpose of DNA extraction Review the main steps in the DNA extraction protocol and the chemistry involved in each step Purpose of DNA Extraction To obtain DNA in a relatively purified form which can be used for further investigations, i.e. PCR, sequencing, etc Basic Protocol Most DNA extraction protocols consist of two parts A technique to lyse the cells gently and solubilize the DNA Enzymatic or chemical methods to remove contaminating proteins, RNA, or macromolecules In plants, the nucleus is protected within a nuclear membrane which is surrounded by a cell membran