桃小食心虫滞育过程中5种代谢酶活力变化.docVIP

桃小食心虫滞育过程中5种代谢酶活力变化 　　摘 要：为明确桃小食心虫越冬适应机制，室内利用试剂盒测定了不同滞育时间桃小食心虫滞育幼虫的海藻糖酶、山梨醇脱氢酶、己糖激酶、丙酮酸激酶和碱性磷酸酶的活力。结果表明，不同滞育时间桃小食心虫体内海藻糖酶、己糖激酶、丙酮酸激酶、碱性磷酸激酶活力差异显著，而山梨醇脱氢酶活力差异不显著。海藻糖酶活力在滞育初期最高，而后随着滞育时间的延长逐渐降低;己糖激酶活力在滞育初期较低，在滞育后期先迅速升高再迅速下降;丙酮酸激酶在滞育中期活力最高，而碱性磷酸激酶活力在滞育初期最高。这表明桃小食心虫在滞育过程中通过代谢酶的调节，提高其越冬期间的抗寒能力，保证顺利越冬。 　　关键词：桃小食心虫;越冬;滞育;代谢酶 　　中图分类号：S436.621.2+9 文献标识号：A 文章编号：1001-4942（2015）06-0097-04 　　Activity Changes of Five Metabolic Enzymes in 　　Carposina sasakii Matsumura during Diapause 　　Zhang Shunyi1，2， Qin Huawei2， Ren Fengshan2， Liu Tingli2， 　　Chen Zhenzhen1， Men Xingyuan2， Yu Yi2， Xu Yongyu1*， Li Lili2* 　　（1. College of Plant Protection， Shandong Agricultural University， Taian 271018， China;2. Institute of Plant Protection， 　　Shandong Academy of Agricultural Sciences/ Key Laboratory for Plant Virology of Shandong， Jinan 250100， China） 　　Abstract To clarify the physiological adaptation mechanisms of overwintering， the activities of 5 metabolic enzymes， including trehalase， sorbitol dehydrogenase， hexokinase， pyruvate kinase and alkaline phosphatase， of diapause larvae of Carposina sasakii Matsumura during different diapause time were measured in laboratory by kit. The results showed that the activities of trehalase， hexokinase， pyruvate kinase， alkaline phosphatase had significant differences between different diapause time， while those of sorbitol dehydrogenase had no significant difference. The trehalase activity was the highest in early diapause， then gradually decreased with the prolongation of diapauses duration. The hexokinase activity was lower in early diapause， then increased rapidly and decreased rapidly in later diapause. The pyruvate kinase activity reached the highest level in mid-diapause. The highest activity of alkaline phosphatase appeared in early diapause. These indicated that Carposina sasakii Matsumura improved their cold tolerance by regulating metabolic enzymes during diapause to ensure overwintering smoothly. 　　Key words Carposina sasakii;