解释质粒图谱.doc

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解释质粒图谱.doc

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解释质粒图谱

质粒图谱信息一.九种表达载体Pllp-OmpA, pllp-STII, pMBP-P, pMBP-C,??pET-GST, pET-Trx, pET-His, pET-CKS, pET-DsbA二.克隆载体pTZ19R DNApUC57 DNAPMD18TPQE30pUC18pUC19pTrcHisA??pTrxFus??pRSET-A??pRSET-B??pVAX1PBR322pbv220pBluescript II KS (+)L4440??pCAMBIA-1301??pMAL-p2X??pGD926三.PET系列表达载体Protein Expression raquo; Prokaryotic Expression raquo; pET Dsb Fusion Systems 39b and 40bProtein Expression raquo; Prokaryotic Expression raquo; pET Expression System 33bProtein Expression raquo; Prokaryotic Expression raquo; pET Expression SystemsProtein Expression raquo; Prokaryotic Expression raquo; pET Expression Systems plus Competent CellsProtein Expression raquo; Prokaryotic Expression raquo; pET GST Fusion Systems 41 and 42Protein Expression raquo; Prokaryotic Expression raquo; pET NusA Fusion Systems 43.1 and 44Protein Expression raquo; Prokaryotic Expression raquo; pET Vector DNAProtein Purification raquo; Purification Systems raquo; StrepTactin Resins and Purification Kits四.PGEX系列表达载体pGEX-1 I/BAP??(T EcoRpGEX-2T pGEX-2TK??pGEX-3X??pGEX-4T-1??pGEX-4T-2??pGEX-4T-3??pGEX-5X-1??pGEX-5X-2??pGEX-5X-3??pGEX-6P-1??pGEX-6P-2??pGEX-6P-3??五.PTYB systemPTYB1PTYB2PTYB11PTYB12六.真核表达载体pCDNA3.1(-)pCDNA3.1(+)pPICZ alpha A??pGAPZαA??PYES2.0pBI121pEGFP-N1? ?pEGFP-C1pPIC9K??pPIC3.5K如何阅读分析质粒图谱质粒图谱信息一.九种表达载体Pllp-OmpA, pllp-STII, pMBP-P, pMBP-C,??pET-GST, pET-Trx, pET-His, pET-CKS, pET-DsbA二.克隆载体pTZ19R DNApUC57 DNAPMD18TPQE30pUC18pUC19pTrcHisA??pTrxFus??pRSET-A??pRSET-B??pVAX1PBR322pbv220pBluescript II KS (+)L4440??pCAMBIA-1301??pMAL-p2X??pGD926三.PET系列表达载体Protein Expression raquo; Prokaryotic Expression raquo; pET Dsb Fusion Systems 39b and 40bProtein Expression raquo; Prokaryotic Expression raquo; pET Expression System 33bProtein Expression raquo; Prokaryotic Expression raquo; pET Expression SystemsProtein Expression raquo; Prokaryotic Expression raquo; pET Expression Systems plus Competent CellsProtein Expression raquo; P