乙脑NS1蛋白与病毒产量的研究讲解.ppt

* Virus-Specific Cytolytic Antibodies to Nonstructural Protein 1 ofJapanese Encephalitis Virus Effect Reduction of VirusOutput from Infected Cells * Contents Ⅰ Background Ⅱ Ⅲ Methods and results Discussion Ⅳ Abstract * * Abstract Sera containing antibodies to NS1 but not those with antibodies to other JEV proteins, such as envelope, brought about complement-mediated lysis of JEV-infected BHK-21 cells. Mouse anti-NS1 cytolytic sera caused a complement-dependent reduction in virus output from infected human cells. Antibodies elicited by JEV NS1 did not cross lyse West Nile virus- or dengue virus-infected cells despite immunoprecipitating the NS1 proteins of these related flaviviruses. JEV NS1 failed to bind complement factor H, in contrast to NS1 of WestNile virus, suggesting that the NS1 proteins of different flavi-viruses have distinctly different mechanisms for interacting with the host. * Background NS1, a glycoprotein, is perhaps the most versatile among these,being involved both in vital processes . The ability of DENV NS1 to bind host complement pointed to a role for this protein in DENV pathogenesis. Virology , 1970 ; J. Virol , 1970 Serum NS1 levels in both DENV and WNV patients correlate directly with disease severity. J. Clin. Microbiol , 2002 ; J. Virol , 2005 Recent studies with WNV NS1 demonstrated its ability to bind human complement factor H, suggesting a role in reducing the host’s ability to bring about complement-mediated control of early virus replication. Proc. Natl. Acad. Sci. USA ,2006 * Background Several studies have pointed to a role for flavivirus NS1-specific immune responses in protection against flaviviruses. Studies by different groups have shown that active immunization with purified NS1 or passive immunization with MAbs against YFV and DENV NS1 provides protection from lethal viral challenge in the absence of neutralizing antibodies. J. Virol , 1986 ;J. Gen. Virol , 1988 ; Virology ,1993 * Background