禾谷缢管蚜和麦长管蚜3小时玻璃管药膜法敏感度毒力基线的建立探索.docVIP

禾谷缢管蚜和麦长管蚜玻璃管药膜法敏感毒力基线的建立 中国农业大学昆虫学系， 北京 100193 摘要:目的】建立禾谷缢管蚜Rhopalosiphum padi和麦长管蚜Sitobion avenae对常用杀虫剂的相对敏感基线。方法】从田间采集麦蚜在实验室内饲养30代以上，利用玻璃管药膜法测定其对杀虫剂的敏感度，每条毒力基线为2次以上独立测定数据合并后计算结果。【结果】用玻璃管药膜法建立了包括新烟碱类、吡啶类、氨基甲酸酯类、有机磷类和拟除虫菊酯类等共22个药剂品种对禾谷缢管蚜和麦长管蚜3 h的敏感毒力基线。禾谷缢管蚜对新烟碱类药剂吡虫啉和啶虫脒的LC50值分别为0.02 g/cm2和0.007 g/cm2；对吡啶类药剂吡蚜酮的LC50值为0.124 g/cm2；对氨基甲酸酯类药剂丁硫克百威、硫双灭多威、灭多威、抗蚜威、西维因的LC50值为0.0026~0.70 g/cm2；对有机磷类药剂三唑磷、丙溴磷、氧乐果、乐果、马拉硫磷、辛硫磷、敌敌畏、毒死蜱的LC50值为0.005~0.065 g/cm2；对拟除虫菊酯类药剂三氟氯氰菊酯、高效氯氰菊酯、溴氰菊酯、联苯菊酯、氰戊菊酯、氯氰菊酯的LC50值为0.033~0.240 g/cm2。麦长管蚜对新烟碱类药剂吡虫啉和啶虫脒的LC50值分别为0.15 g/cm2和0.12 g/cm2；对吡啶类药剂吡蚜酮的LC50值为0.41 g/cm2；对氨基甲酸酯类药剂丁硫克百威、硫双灭多威、灭多威、抗蚜威、西维因的LC50值为0.005~0.76 g/cm2；对有机磷类药剂三唑磷、丙溴磷、氧乐果、乐果、马拉硫磷、辛硫磷、敌敌畏、毒死蜱的LC50值为0.018~0.36 g/cm2；对拟除虫菊酯类药剂三氟氯氰菊酯、高效氯氰菊酯、溴氰菊酯、联苯菊酯、氰戊菊酯、氯氰菊酯的LC50值为0.20~2.94 g/cm2。【结论】建立的两种麦蚜对22杀虫药剂的相对敏感基线，包括了当前所有可能用于防治麦蚜药剂，可以用于以后麦蚜抗药性监测或其他相关研究的参照；禾谷缢管蚜对药剂的敏感度高于麦长管蚜。 关键词:禾谷缢管蚜；麦长管蚜；玻璃管药膜法；敏感毒力基线；抗药性监测 Q965.9 文献标识码：A 文章编号：0454-6296 2009 01-00-00 Establishment of baseline susceptibility data to various insecticides for aphids Rhopalosiphum padi（Linnaeus）and Sitobion avenae（Fabricius）by the method of residual film in glass tube LU Yan-Hui, YANG Ting, GAO Xi-Wu （Department of Entomology, China Agricultural University, Beijing 100193, China） Abstract：【】 The objective of this research was to establish susceptible toxicity baselines of both Rhopalosiphum padi and Sitobion avenae to 22 insecticides. 【Method】 The method of the residual film in glass tube with 3 h exposure to insecticides was employed for determining toxicity of insecticide to both wheat species, which were originally collected from different provinces of China during 2005 2007 and reared on wheat seedlings in laboratory for more than 30 generations. 【Result】 n R. padi, the LC50 values of susceptible toxicity baselines were 0.02 μg/cm2 and 0.007 g/cm2 for neonicotinoid insecticides, imidacloprid and acetamiprid respectively; 0.124 g/cm2 for pymetrozin; 0.0026-0.70 g/cm2 for carbamate insecticides, carbos