多效唑对百子莲花葶矮化效应的研究讲解.doc

多效唑对百子莲花葶矮化效应的研究 卓丽环1王会1 张凤娥1 （1.上海农林职业技术学院上海20160；2.东北林业大学园林学院哈尔滨150040） 摘要：以三年生盆栽百子莲（Agapanthus praecox ssp. rientalis ‘Big Blue’）为试材，研究不同浓度的多效唑对百子莲花葶生长、细胞形态和生理指标的影响。结果表明：不同浓度的多效唑对百子莲花葶长和细胞纵向生长均有不同程度地抑制作用，且随着多效唑浓度的，其中以600mg/L的多效唑最显著，花葶长度和细胞长度分别比对照平均缩短了70.2%和59.1%花葶长度与其上下部细胞长度极显著正相关，相关系数为0.772和0.806由切片组织化学分析结果表明，多效唑处理明显提高了花葶中可溶性蛋白、可溶性糖和淀粉含量。关键词：百子莲；多效唑；花葶；细胞形态 Effects of Paclobutrazol on Dwarfing in Scape of Agapanthus praecox ssp.rientalis ‘Big Blue’ Zhuo Lihuan 1, Wang Hui 2, Xin Yafen 1,Zhang Fenge1 1. Shanghai Vocational and Technical College of Agriculture and Forestry，Songjiang，Shanghai，201600；2. College of Landscape and Architecture，Northeast Forestry University，Harbin，Heilongjiang，150040）Abstract：Three-year-old Agapanthus praecox ssp. orientalis ‘Big Blue’ was used as experimental sample to investigate the effects of different concentrations of PP333 on their scape development and growth, cell morphology and physiological index. The results indicate that different concentrations of PP333 can effectively inhibit the scape elongation and cell growth, and the length of scape decreased with the concentration of PP333 increasing. 600 mg/L PP333 has the most significant effect on dwarf of scape, and the lengths of scape and cell were less 70.2% and 59.1% than CK. Scape length and the upper and lower parts of cells length have significantly positive correlation, and the correlation coefficients are 0.772 and 0.806. In this study, we also found that PP333 can enhance the soluble sugar, starch and soluble protein concentrations in scape. Key words：Agapanthus praecox ssp. Orientalis；PP333；scape；cell morphology 百子莲属Agapanthus）原产于南非亚热带地区，是多年生根茎类花卉，叶二列状基生，花葶自叶丛中抽生，挺拔直立，伞形花序，小花数量繁多[1]。因其种类丰富，叶形秀丽，花朵姿态优美，花期持续时间长而逐渐成为人们喜爱的观赏花卉。百子莲自引入中国以来，在上海地区自然生长状态下花葶高0.6～1.2米[2]，是一种良好的鲜切花材料，但受其花葶高度影响，在室内盆栽观赏受到一定限制，为推广百子莲的应用范围，提高其观赏价值，有关百子莲花葶的矮化研究很有必要。 已有研究证明GA3+4/IAA的比值变化与百子莲花葶伸长的发育速度，当IAA含量高促进花器官分化及发育，GA含量提高则促进花葶的伸长，GA3+4/IAA比值高可促进花葶的伸长[3]。多效唑是一种常用的植物生长延缓剂，在观赏植物中已被广泛应用于控制株高、调整株型、促进生