microbialsourcetrackingintwosouthern-maineseagrant.pptVIP

What is Bacterial Pollution? Sources of Bacterial Pollution? Unmanaged livestock/pet waste Leaking sewer pipes/storm overflows Wildlife incl. mammals and birds Malfunctioning septic systems Importance of Bacteria as Water Quality Indicators What is Microbial Source Tracking? Why Use MST? MST Step 1: Intensive Water Sampling MST Step 2: Collect Fecal Samples For Reference Library MST Step 3: Select and Save Bacteria All water samples tested for E. coli Source Species Database Results of MST in Maine: Ribotyping:Lab Procedures DNA extracted purified. DNA digested w/restriction enzyme. DNA separated via gel electrophoresis. DNA denatured blotted onto membrane. Hybridization with E. coli rRNA DNA probe. DNA exposed to a chemiluminescent substrate digitally imaged. Image enhanced optimized in computer. Ribotyping:Data Analysis DNA patterns are analyzed by cluster analysis and by computing a similarity coefficient. Source species identification for sample patterns based on degree of matching to source species patterns. Matching ribotype gel banding patterns: Researcher Sets Similarity Criteria Results of Ribotyping in Webhannet Watershed: Local Source Library Results of Ribotyping in Webhannet Watershed: Regional Source Library CONCLUSIONS Webhannet report being drafted now. Awaiting steering committee input re: management ideas. Results provide data in place of speculation. As more regional MST is conducted, library size should expand and % unknown diminish. Perhaps most useful when one or two major sources are suspected and impact is severe. Driven by need and interest, the basic science underlying MST is being researched further. * NEERS/SNECAFS Joint Meeting, May 9, 2003 Project Author: Kristen Whiting-Grant, Maine Sea Grant  Cayce Dalton*, AmeriCorps/Maine Conservation CorpsFred Dillon*, U Southern Maine-Muskie School for Public Service Steve Jones, Jackson Estuarine Lab, U New HampshireMichele Dionne, Wells National Estuarine Research Reserve* pres